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KMID : 0881720140290020123
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Journal of Food Hygiene and Safety
2014 Volume.29 No. 2 p.123 ~ p.130
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Simultaneous Determination of Aminoglycoside Antibiotics in Meat using Liquid Chromatography Tandem Mass Spectrometry
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Cho Yoon-Jae
Choi Sun-Ju
Kim Myeong-Ae
Kim Mi-Gyeong
Yoon Su-Jin
Chang Moon-Ik
Lee Sang-Mok
Kim Hee-Jeong
Jung Ji-Yun
Rhee Gyu-Sik
Lee Sang-Jae
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Abstract
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A simultaneous determination was developed for 9 aminoglycoside antibiotics (amikacin, apramycin, dihydrostreptomycin, gentamicin, hygromycin B, kanamycin, neomycin, spectinomycin, and streptomycin) in meat by liquid chromatography tandem mass spectrometry (LC-MS/MS). Each parameter was established by multiple reaction monitoring in positive ion mode. The developed method was validated for specificity, linearity, accuracy, and precision based on CODEX validation guideline. Linearity was over 0.98 with calibration curves of the mixed standards. Recovery of 9 aminoglycosides ranged on 60.5~114% for beef, 60.1~112% for pork and 63.8~131% for chicken. The limit of detection (LOD) and limit of quantification (LOQ) were 0.001~0.009 mg/kg and 0.006~ 0.03 mg/kg, respectively in livestock products including beef, pork and chicken. This study also performed survey of residual aminoglycoside antibiotics for 193 samples of beef, pork and chicken collected from 9 cities in Korea. Aminoglycosides were not found in any of the samples.
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KEYWORD
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aminoglycoside antibiotics, meat, validation, LC-MS/MS
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